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KMID : 1161520000040020181
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Animal Cells and Systems
2000 Volume.4 No. 2 p.181 ~ p.186
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Involvement of putative heat shock element in transcriptional regulation of p21WAF1/CIP1/SDI1 by heat shock
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Woo Sang-Hyeok
Oh Su-Young
Han Song-ly
Choi Yung-Hyun
Kang Kwang-II
Yoo Mi-Ae
Kim Han-Do
Kang Ho-Sung
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Abstract
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The expression of p21WAF1/ClP1/SDI1, one of the cyclin?dependent kinase inhibitors, is regulated by a variety of transcription factors including p53 and STAT. Heat shock induces the expression of p21 in a temperature? and time?dependent manner. Although the p21 induction by heat shock has been reported to be controlled by p53, a p53?independent mechanism is also involved. To understand the p53?independent regulation of heat shock?induced p21 expression, we searched the promoter region of p21 gene and found one or two heat shock element (HSE)?like sequences in human, rat, and mouse. Electromobility shift assay (EMSA) showed that heat shock factor (HSF) could bind to these HSE?like sequences in response to heat shock, even though to a lesser extent than to HSE. In addition, p21 promoter deletion analysis revealed that heat shock activated a p21 deletion promoter construct containing the HSE?like sequences but lacking p53?binding sites, but not a promoter construct containing neither HSE?like sequences nor the p53?responsive element. Furthermore, the p21 induction by heat shook was significantly inhibited in confluent cells in which heat shock?induced HSF activation was reduced. These results suggest that the transcriptional regulation of p21 by heat shock may be mediated through activation and binding to HSE?like sequences of HSF.
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KEYWORD
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21WAF1/CIP1/SDI1, heat shock, HSF, HSE-like sequence, p53
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